您好!欢迎光临申琅生物网站
  • 产品
  • 新闻
首页 >>

Q-VD-OPH

参数名
参数值
别名
Z-VAD-FMK
规格
5mg
CAS
187389-52-2
单价:¥-3.0
相关产品
  • Product name

    QVD-OPh, caspase inhibitor

  • Description

    Potent, cell-permeable, irreversible broad-spectrum caspase inhibitor

  • Biological description

    Potent, cell-permeable, irreversible broad-spectrum caspase inhibitor (IC50 values are 25, 50, 100 and 430 nM for caspase-3, 1, 8 and 9 respectively). Inhibits apoptosis. Shows anti-ischemic and neuroprotective effects in vivo. Blood-brain barrier permeable.

  • Purity

    > 95%

  • CAS Number

    1135695-98-5

  • Chemical structure

    Chemical Structure

Properties

Application Notes

Q-VD-OPH is a novel, irreversible, pan caspase inhibitor specifically designed for in vivo and in vitro research. Q-VD-OPH is widely cited in the literature, and researchers should refer to the literature for additional information about the various species that Q-VD-OPH has been used for.For in vitro applications, Q-VD-OPH is typically used at final working concentration of 10-100 uM. The variability depends on model system, including cell type, culture, properties, and type of apoptosis induction treatments. Each researcher should empirically establish optimal working concentrations for their in vitro model system.
For in vivo applications, the recommended dose of Q-VD-OPh is 20 mg/kg. The caspase inhibitor is administered IP in 80-100% DMSO. Doses of up to 120 mg/kg have been used in mice without toxic effects (Melnikov and Vyacheslav, 2002; Patil and Sharma, 2004). Each researcher should empirically establish optimal doses for their animal model system. Use in functional reported in scientific literature

Reactivity Notes

Human reactivity reported in scientific literature

Packaging, Storage & Formulations

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Reconstitution Instructions

Form : Off-white Solid: Make a stock solution of 10 mM in high purity DMSO (>99.9%). Add 195 uL DMSO to 1 mg of the pan caspase inhibitor (Q-VD-OPH peptide) to make a 10 mM stock solution.

Background

Members of the caspase family play key roles in apoptosis and inflammation. The OPH trap of Q-VD-OPH has superior potency, cell permeability, minimal toxicity, and provides an alternative to the fluoromethylketone (FMK) family of inhibitors. Like the FMK caspase inhibitors (reviewed in Thornberry and Lazebnik, 1998; Gregoli and Bondurant, 1999; Schrantz et al., 1999), Q-VD-OPH binds to the catalytic site of caspases proteases, and inhibits caspase-mediated apoptosis by preventing caspase activity. Users should consult the literature for additional information regarding applications for Q-VD-OPH (Caserta et al., 2003; Melnikov et al, 2002; Patil and Sharma, 2004)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Inhibitors are guaranteed for 1 year from date of receipt.


Immunogen

Q-VD-OPH is a cell permeable caspase peptide inhibitor. Caspase inhibitors irreversibly bind to the catalytic site of caspase proteases and inhibit apoptosis. Caspase inhibitors may be broad spectrum, inhibiting multiple caspases, or may preferentially inhibit particular caspases. Z-VAD-FMK, BOC-D-FMK, and Q-VD-OPH are examples of broad spectrum or pan caspase inhibitors. In contrast Z-DEVD-FMK preferentially inhibits caspases 3 and 7, Z-IETD-FMK preferentially inhibits caspase 8, Z-LEHD-FMK preferentiailly inhibits caspase 9. Z-FA-FMK is considered to be a negative control for FMK based caspase inhibitors.

Details of Functionality

Mass Spec: M+1=514.1

Chromatography: TLC:Rf: 0.4 Single Spot, EtOAC:6, ACOH:0.1

H NMR: All functional groups are present

Content

Q-Val-Asp(non-omethylated)-OPH, also known as Q-VD(non-omethylated)-OPH. Molecular Weight: 513


新手入门
用户指南
会员说明
联系我们
订购指南
订购流程
订购询价
订单处理
常见问题
支付方式
货到付款
线上支付
银行转账
配送说明
物流说明
商品验货
售后服务
退换货说明
退换货流程
退款说明
帮助中心
用户注册
找回密码
联系客服
商务合作